LABORATORY EXERCISE #10

KINGDOM PLANTAE

 

THE VASCULAR PLANTS - I

 

Introduction

 

Vascular plants include most of the plant species with which we are familiar.  The plants which make up a majority of our food, the forest trees from which our dwellings are made, the plants which furnish the fibers of which our clothes are constructed, all are vascular plants.  So also are the shrubs, which surround our homes, the delicate flowers that brighten our lives, and the logs crackling in the fireplace.  The distinguishing characteristic of this group is its possession of vascular tissue.  This tissue serves the plants in much the same way as the animal circulatory system, delivering materials from one part of the plant to another.

 

The two types of vascular tissue found in plants differ in appearance and function.  The xylem (pronounced "zy lum") conducts water and dissolved minerals from the sites of their absorption in the roots, to the remainder of the plant.  It also aids in the mechanical support of the plant.  Phloem ("flow um"), on the other hand, moves food materials from the site of formation or storage, to other parts of the plant.

 

 

ACTIVITIES

 

a.  Select a slide marked "Dicot and Monocot Root", examine under low power and find the specimen that resembles the photographs of Figures 75a and c in your Photo Atlas.  Focus on the circle of cells in the center and locate the xylem and phloem.  The large, thick-walled xylem vessels usually take on a red stain, while the thin-walled phloem cells are greenish-blue.

 

 

Diversity Among The Vascular Plants

 

 

There are three major groups of vascular plants.

 

1.  The primitive vascular plants, which do not form seeds, include the ferns, club mosses and horsetails.  These taxa have life cycles in which the sporophyte generation is predominant, unlike the mosses studied previously.  The gametophyte generation, though diminutive, is none-the-less free living.  Like the mosses, these plants reproduce by the use of spores, rather than seeds. All three groups have long fossil histories and once dominated the land.  Those species existing today hang on in rather specialized ecological niches.


 

ACTIVITIES

 

b.  Consider the herbarium specimens of the ferns.  These plants typically have an underground stem with many small, wiry roots and comparatively large leaves or fronds.  Aided by Atlas Figures 51a and b, examine the fronds and look for brownish spots on the underside of some leaflets.  These are the fruit dots and bear the spores; consequently, this plant is a sporophyte.  Note the diversity in size and shape among the ferns.

 

c.  Select a slide of the fern prothallus (gametophyte).  This tiny, heart-shaped organism is shown in Figure 53a.  It bears the male reproductive structures (antheridia), as well as the female organs (archegonia).  Also present on the prothallus are tiny, root-like rhizoids.  Locate all these structures, using Figures 53a and b to assist you.

 

d.  Examine the herbarium specimens of club mosses and compare them to the photographs in Figures 48a-c in your Photo Atlas.  These are the last of a proud group, which dominated the forests of the earth during the Carboniferous Period, some 350 million years ago.  Those ancestral club mosses were large trees, up to 150 feet high.  Much of the coal mined today was formed from the remains of these swamp-dwelling plants.  A fossil club moss is available for your examination.

 

e.  Also inhabiting those Carboniferous swamps were large, leafless plants ten meters high and as big around as a man's thigh.  The descendants of those organisms are the horsetails.  Only one genus and about a half dozen species exist today, but they are never the less interesting plants.  Look at the herbarium specimen and/or plastomounts of horsetails, as well as the photographs on page 50 of Perry and Morton.  The leaves have been reduced to whorls of scales and the green stem has taken over the job of photosynthesis.  The epidermis of these plants contains high levels of silica, a rough and abrasive mineral, leading to their being used as scouring pads by pioneer women.

 

 

 

2.  The gymnosperms are primitive seed plants which are present in abundance today and which are very important to modern humans.  The cone-bearing trees (conifers) are a part of this group.  They include the oldest, tallest and bulkiest living organisms (bristle-cone pines, redwoods and sequoias, respectively).  Most of the timber used in building construction comes from such conifers as pine, fir, cedar and redwood, and we enjoy using the beautiful evergreen gymnosperms for ornamental plantings.

 

The sporophyte generation dominates the gymnosperm life cycle; indeed, the gametophyte has been reduced to only a few cells and there are two types, male and female, both of which usually form on the same tree.  Seeds which are not formed inside a fruit also bring about reproduction.  The name gymnosperm means "naked seed," a reference to the small seeds of the conifers which lie naked upon the scales of the reproductive structure or cone.

 

All gymnosperms are woody plants.  In addition to the conifers, the cycads (Figure 54a and b) and ginkgos (Figures 54c and d) make up the group.


 

ACTIVITIES

 

f.  Examine the herbarium mounts of conifers.  Note the woody stems, the needle-like or scale-like leaves and the cones.  Remember, this is part of a sporophyte.

 

g.  Look at the representative cones presented.  Do you suppose there is a direct correlation between the size of the tree and the size of its cones?  Actually there is not.

 

 

 

3.  The angiosperms are the flowering plants.  The reproductive structure of the angiosperm is the flower and the seeds are protected by the thickened wall of the ovary, within which they form and mature.  This "ripened" ovary, with its seeds, is termed a fruit.  The angiosperms include large forest trees with their woody supporting stems, as well as the smaller herbaceous plants which lack wood.

 

It is difficult to over-emphasize the importance of angiosperms in today's world.  Most of the mid-latitude and tropical forests are made up almost exclusively of "hardwood" trees which belong to this group.  Basically all of the plant materials we eat and/or feed to domestic animals are the products of angiosperms.  Most birds, mammals and insects find home and sustenance among these plants, and we enjoy the sight and smell of their flowers and languor in their shade.  Can you imagine a world without this group of plants?

 

The angiosperms are divided into two major groups, based on a few easily-identified characteristics.  Illustrated in Figure 22.9, p. 381 in Starr, these are:

 

A.  The monocots are all herbaceous.  Their flower parts are in threes or multiples of three, and a cross-section of the stem reveals scattered bundles of vascular tissue.  The leaves of monocots have the vascular tissue confined to many parallel veins.  Included in this group are the lilies, the orchids and the grasses.

 

B.  The dicots may be herbaceous or woody.  Their flower parts are in fours or fives or multiples of these.  Their vascular bundles are arranged in a ring, and the veins of the leaves form a network.  All of the hardwood trees are dicots, as are many crop plants such as beans, tomatoes, cabbage, etc., and a majority of the spring flowers.

 

Angiosperms may complete an entire life cycle in one year, over-wintering as seed (annual plants), or they may live for many years (perennials).  Some woody angiosperms are evergreen (southern magnolia, for instance), while others are deciduous, losing all their leaves in the Fall and replacing them in Spring.


 

ACTIVITIES

 

h.  Examine any live specimens of angiosperms.  Note the division of the plant body into roots, stem and leaves.  If flowers are present, note their location.  Using the criteria listed above, and assisted by Photo Atlas Figures 59a and b, can you identify the plant as a monocot or dicot?  Is it woody or herbaceous?

 

i.  Examine the herbarium specimens of monocots.  Are they woody or herbaceous?  Note the parallel veins of the leaves.

 

j.  Look at the preserved specimens of dicots.  Are they herbaceous or woody?  Compare the net-veined leaves seen here with the parallel veins of the monocots.

 

 

 

The Vascular Plant Body

 

The typical vascular plant is made up of the roots, stems and leaves (See Figure 22.2, p. 378 in Starr).  There are many modifications of these basic parts and the varying combinations give rise to the infinite variety which exists in the plant kingdom.  The remainder of this laboratory period is given to examining some of this variety.

 

1.  The roots typically constitute that part of the plant which lies below ground level.  The roots' primary functions are to anchor the plant in its substrate, absorb water and mineral nutrients from the soil and store food materials.  Toward those ends, plants have evolved two types of root systems, both of which are illustrated in Figure 22.15 on page 386 of Starr.  In some plants, the grasses for example, the root system consists of a tangle of many equal-sized parts.  These are termed fibrous roots.  Others, like the carrot, have a single dominant root which grows straight down from the stem.  While there may be several smaller roots as well, this tap root dominates.

 

 

ACTIVITIES

 

k.   Look at the plastomounts illustrating root types.  Be able to distinguish between fibrous roots and tap roots.

 

l.   Examine a slide of root hairs.  Using Figure 74a in Perry and Morton, locate the root cap.  This mass of cells protects the growing tip of the root by lubricating its passage through the soil and by sacrificing its own cells to save the delicate tissue behind it.  Some distance behind the root cap you may observe the stubs of root hairs which are out growths from some epidermal cells.  It is in the root hair region that most water and minerals are absorbed by the root.

 

 

 

  1. The main axis of the plant which projects above the ground is termed the shoot and the central portion of this is the stem.  The chief functions of the stem are support and storage, but in some cases it is also an important organ of photosynthesis.  In some plants the stem is made up primarily of thin-walled cells which maintain their rigidity only when pumped full of water.  In these herbaceous plants, a lack of adequate water results in the plants "wilting".  Other plants have stems with much xylem, the cell walls of which are thickened with cellulose and other materials.  These woody stems remain erect in the face of drought and even after the death of the plant.  The wood making up our lab furniture is simply xylem tissue.

 

ACTIVITIES

 

m. Examine one of the twigs presented and, using Figures 78c and d in your Photo Atlas, identify the terminal bud, lateral buds, and leaf scars.  The scars left by past years' terminal buds are visible as rings around the twig and are termed nodes.  The space between two nodes represents one year's growth.  How many years growth can you count on the twig you are examining?

 

n.  Select a slide of Monocot and Dicot stems.  Use the photomicrographs on page 77 in Perry and Morton to help you identify which is which.  In the monocot (probably corn) the vascular bundles are more or less scattered around the periphery of the stem.  Note the "monkey face" look of the vascular bundles, owing to the large xylem vessels.  The dicot stems, on the other hand, lack the "monkey face" in the vascular bundles, which are arranged in a definite ring around a large mass of pith.

 

 

3. On most plants, parts of the shoot have been expanded and flattened into special photosynthetic organs called leaves.  Some leaves may be thickened to also serve as a site for storage of food and/or water, and others may be modified to protect the plant, as in the case of cactus spines.  The leaf is divided into a stem and the expanded, flattened portion; the blade.  The veins of the blade contain the vascular tissues.  While the leaves of angiosperms are flattened, those of gymnosperms are typically divided into thin needles.

 

ACTIVITIES

 

o. Examine the herbarium specimens showing the leaves of both angiosperms and gymnosperms.  Can you tell the difference?  Aided by Starr Figure 22.11 (p. 382), be able to differentiate between simple and compound leaves.  Your instructor will assist you.